Co-Modification of Beta-Catenin and Sex Determining Region Y-Box9 Genes Regulates the Chondrogenesis of Human Adipose Tissue Derived Stem Cells

Beta catenin gene can inhibit the chondrogenic differentiation of human adipose tissue derived stem cells, while sex determining region Y-box9 promote cells. However, it is still necessary to study role combination two genes in early The purpose this was investigate whether knockdown and overexpression combined with modification cells could Therefore, divided into four groups or lentivirus vectors, including negative control group (empty vector), Beta-catenin (lentivirus-RNA interference-Betacatenin), (lentivirus-FLAG-sex Y-box9) union interference-Beta-catenin+lentivirus-FLAG-sex Y-box9). After 24 h transfection, quantitative reverse transcription Polymerase Chain Reaction Western blot were used detect efficiency gene. Furthermore pellets cultured medium for 21 d stained safranin-O-green toluidine blue. messenger RNA expression cartilage related detected by polymerase chain reaction. Quantitative reaction results showed that both successfully transfected expressed (p< 0.05). type-II collagen alpha 1 aggrecan levels higher than those first three (p<0.05), type-X type-I lower (p<0.05). staining absorbance former Immunohistochemical protein